Identification of blaOXA23 and blaNDM1 from Carbapenem- resistant Acinetobacter baumannii at a Private Hospital in Thailand

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Date:
2019
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Bangkok Dusit Medical Services (BDMS) Foundation
Abstract:
OBJECTIVES: This study aimed to detect carbapenemase genes and their clonal relationships among carbapenem-resistant Acinetobacter baumannii (CRAB) clinical isolates.
MATERIAL AND METHODS: Fifteen CRAB isolates were collected from patients admitted to Phyathai II International Hospital, Bangkok, Thailand during August 2014 – April 2015. Polymerase chain reaction (PCR) amplification and DNA sequencing were used to identify blaOXA23, blaOXA40, blaOXA48, blaOXA58, blaIMP, blaVIM, blaKPC, and blaNDM. Clonal relationships were explored by using repetitive element palindromic (REP)-PCR.
RESULT: The CRAB isolates were categorized by REP-PCR in 8 groups [A-H], with 53.3% belonging to group A, whereas the remaining 7 clones were in each member of B-H, respectively. The blaOXA23 was detected in most CRAB isolates (86.7%) whereas only two isolates harbored blaNDM1 with blaOXA23 (13.3%).
CONCLUSION: Most CRAB strains carried blaOXA23 as reported in several related studies but our finding confirmed the emergence of CRAB carrying multiple types of carbapenemase genes in Thailand. This is a worrying phenomenon that concerns the spread of such CRAB genotypes
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Published in : The Bangkok Medical Journal Vol. 15 No. 2 (2019): September ; p.149-153
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มหาวิทยาลัยศิลปากร
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